EN 1500 Hygienic Handrub Method

The EN 1500 is a European Standard test method that evaluates the efficacy of a hygienic handrub by measuring the number of viable bacteria remaining on the fingertips after contamination and handrub exposure. A handrub is defined as a treatment that involves rubbing the hands without the addition of water. This method specifically simulates conditions for establishing if a hygienic handrub decreases the release of transient flora from the hands.

EN 1500: PROCEDURE AT A GLANCE

In this test, subjects with healthy hands are randomly assigned either a reference control product or the hygienic handrub under evaluation. Following an initial cleansing wash with soft soap to remove natural transient microorganisms, the subject's hands are dried thoroughly with a paper towel.

Typically a prepared pure culture of a non-pathogenic strain of Escherichia coli is used as the inoculum for this test method. Subjects immerse their hands in a volume of the inoculum, up to the mid-carpals, for 5 seconds with their fingers spread apart. The hands are then allowed to air dry for 3 minutes, then the fingertips are sampled by rubbing them into a petri dish with sterile tryptic soy broth (TSB) for pre-values of viable bacteria present on the hands.

Immediately after pre-value sampling the hands are re-inoculated, then subjected to either the reference handrub procedure with propan-2-ol 60% (v/v), or the hygienic handrub being tested per instructions provided by the manufacturer. The fingertips are then sampled again for post-values in the same manner as the pre-values, this time with TSB containing a chemical neutralizer. The samples are then diluted appropriately and plated onto tryptic soy agar (TSA) medium, where they are allowed to incubate 18-24 hours at 36 ± 1ºC, counted, then re-incubated for an additional 24 hours to detect any potential slow growing colonies.

The pre- and post-values recovered from the fingertips are evaluated against one another, resulting in a ratio called the reduction factor. The reduction factor provides a quantitative measure of the antimicrobial efficacy. This is determined by comparing average counts of viable microorganism colony forming units of the pre-values to the average counts after exposure to the hygienic handrub.

En 1500: VIVO CLINICAL TESTING’S EXPERTISE

Vivo Clinical Testing was founded in 2017 by Dr. Benjamin Tanner, a respected microbiologist and owner of Microchem Laboratory, and is staffed by many experienced Microchem Laboratory employees.  Vivo is committed to providing the same high testing standards that have been demonstrated by Microchem Laboratory since 1988.

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An FDA GLP and GCP-compliant testing organization staffed by skilled, experienced microbiologists and subject recruiters.  Vivo can help speed your project to completion.

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